Showing: 1221 - 1240 of 1875 results
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Plasmid#79619PurposeExpresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTags3xFlag-PmCDA1ExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79621PurposeExpresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionMammalianMutationD10A and H840A for nuclease deficient Cas9PromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
Semaphorin7A-CD4d3+4-bio
Plasmid#73115PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bioDepositorInsertSemaphorin 7A (SEMA7A Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNICKclos2.0
Plasmid#73228PurposeGenome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052DepositorInsertsCas9 nickase
sgRNA to xylR
UseE.coli - clostridium shuttle vectorTagsExpressionMutationD10APromoterPj23119 and PthlAvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMutationhuman codon-optimized, D10A + H840APromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
Stargazin-GFP-LOVpep
Plasmid#80406PurposeMembrane localized GFP-tagged LOVpep. Expresses in mammalian cellsDepositorInsertStargazin-GFP-LOVpep (CACNG2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
ANKRD24-TwinStrepII
Plasmid#83190PurposeExpression of tagged protein in mammalian cellsDepositorInsertANKRD24 (Ankrd24 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LAMP1
Plasmid#110948PurposeExpresses LAMP1 fused to mTurquoise2DepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailabilityAcademic Institutions and Nonprofits only -
pJMP1339
Plasmid#119271Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailabilityAcademic Institutions and Nonprofits only -
pJMP1341
Plasmid#119272Purposecontrol sgRNA in cells lacking rfpDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH
Plasmid#112211PurposeTargeted DNA methylationDepositorInsertC-terminus of dCas9
UseAAVTagsExpressionMutationD10APromoterAvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-Sumo-TEV human OAS1
Plasmid#130895PurposeHuman oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobosDepositorInsertoligoadenylate synthase 1 (OAS1 Human)
UseTagsStrep_SumoExpressionInsectMutationPromoterphAvailabilityAcademic Institutions and Nonprofits only -
pRS0035
Plasmid#131125PurposeMammalian Target-AID expression vectorDepositorInsertnCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1 and UGIExpressionMammalianMutationD10A in SpCas9PromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVneo
Plasmid#134982PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:dCas9:nls:10P3
Plasmid#135982PurposeExpresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cellsDepositorInsertdCas9 fused to 10 copies of the P3 coiled-coil
UseCRISPRTagsHisExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1221 - 1240 of 1875 results