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pv7_CBX1_2xChromo_noEGFP

Plasmid

#179395

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo_noEGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo-W35A

Plasmid

#179397

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo(W35A)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_CBX7_Chromo-W35A_TAF3_Phd

Plasmid

#179401

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo(W35A) - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

Plasmid

#179402

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd(DW890/891AA)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Phd domain DW890/891AA

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_MBD1_MBD-R22A

Plasmid

#179405

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD(R22A)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R22A

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
pv7_MBD1_MBD_noEGFP

Plasmid

#179406

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p28BIOHTEV-LIC: nsp10_SARS-CoV-2

Plasmid

#167847

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Industry, Academic Institutions, and Nonprofits

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX chimerin2-SH2

Plasmid

#46416

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin2 (CHN2 Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 49-155

Promoter

Tac

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Use

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX FynA-SH2

Plasmid

#46429

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynA (FYN Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX FynT-SH2

Plasmid

#46430

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynB/FynT (FYN Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Mutation

Promoter

Human EF1a

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD

Plasmid

#179407

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_MECP2_MBD-R106W

Plasmid

#179408

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R106W) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R106W

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

Enlarge