Skip to main content
Addgene
Showing: 3051 - 3100 of 30014 results

  2. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

    Enlarge

  3. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

    Plasmid
    #110831
    Purpose
    Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    Azgp1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110831-plasmid-map-sequence-id-214111

    Enlarge

  11. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

    Enlarge

  12. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid
    #163359
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    163359-plasmid-map-sequence-id-322106

    Enlarge

  17. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid
    #72608
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine37 to alanine
    Promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. 1070 pBabe puroL PTEN 360-364A A4

    Plasmid
    #10943
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…
    Promoter
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. integrin alpha9 / alpha5 EGFP pBabe puro

    Plasmid
    #13608
    Depositor
    Dean Sheppard
    Insert
    integrin alpha 9 / alpha 5 (ITGA9 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Integrin alpha 9 chimera containing the cytoplasm…
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only
    13608-plasmid-map-sequence-id-254609

    Enlarge

  24. 1038 pBabe puroL PTEN 360-364A

    Plasmid
    #10940
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    360-364A (SSSTS to AAAAA)
    Promoter
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  34. Anti-Neuroligin-1 [N97A/31-rat IgG2a] - Chimeric

    Plasmid
    #227064
    Purpose
    Mammalian expression plasmid of anti-Neuroligin-1 (Rattus norvegicus) rat IgG2a R-mAb. Derived from hybridoma N97A/31.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Nlgn1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227064-plasmid-map-sequence-id-450781

    Enlarge

  35. Anti-Neuroligin-2 [L107/39-rat IgG2a] - Chimeric

    Plasmid
    #227065
    Purpose
    Mammalian expression plasmid of anti-Neuroligin-2 (Mus musculus) rat IgG2a R-mAb. Derived from hybridoma L107/39.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody. (Nlgn2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227065-plasmid-map-sequence-id-452555

    Enlarge

  36. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

    Enlarge

  37. pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

    Plasmid
    #197902
    Purpose
    For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.
    Depositor
    Bo Wang
    Article
    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:
    Insert
    HSALNG0104472-163 (HSALNG0104472-scramble-sequence)
    Use
    Lentiviral
    Tags
    zsGreen1
    Expression
    Mammalian
    Mutation
    15q11.2 deletion
    Promoter
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    197902-plasmid-map-sequence-id-389136

    Enlarge

  38. pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

    Plasmid
    #216666
    Purpose
    Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    MyoD (Myod1 Mouse)
    miRFP703
    PuroR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216666-plasmid-map-sequence-id-432196

    Enlarge

Showing: 3051 - 3100 of 30014 results