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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRM3-cpHalo∆-mEGFP

Plasmid

#194678

Purpose

CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

CHRM3_cpHalo∆

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al bioRxiv 2023.09.08.556841

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Use

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Use

Tags

6x His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ hnRNPK myc HIS

Plasmid

#71662

Purpose

Mammalian expression of hnRNPK

Depositor

Gideon Dreyfuss

Insert

hnRNPK (HNRNPK Human)

Use

Tags

HIS and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsStaufen2-T1_V

Plasmid

#147741

Purpose

Mammalian Expression of HsStaufen2-T1

Depositor

Elisa Izaurralde

Insert

HsStaufen2-T1 (STAU2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector

Plasmid

#60648

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) WT V5 His

Plasmid

#119287

Purpose

CIP2A WT V5 overexpression

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Flag-His-ATM kd

Plasmid

#31986

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM kd (ATM Human)

Use

Tags

Flag and His

Expression

Mammalian

Mutation

3'UTRf 9 nucl/D2870A N2875k. catalytically …

Promoter

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

Plasmid

#183252

Purpose

Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

anti-CD19 scFvFc (FMC-63)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(WT)-EGFP

Plasmid

#89633

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)

Plasmid

#193995

Purpose

plasmid encoding myc-tagged OGA(GS-544)

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-BLIMP-1-pcDNA3.1-

Plasmid

#52518

Purpose

expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blimp1 (PRDM1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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