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p667-UBC-PRKCQ-V5-miniTurbo_IDG-K

Plasmid

#170590

Purpose

Gateway destination clone of PRKCQ (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PRKCQ-V5-miniTurbo (PRKCQ Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PKN3_IDG-K

Plasmid

#170601

Purpose

Gateway destination clone of PKN3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PKN3 (PKN3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Hs.HRAS Q61R

Plasmid

#83188

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61R

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-2.3k-fabp2

Plasmid

#84927

Purpose

–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector

Depositor

John Rawls

Insert

–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)

Use

Tags

NO

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-APN

Plasmid

#158440

Purpose

Gateway Cloning compatible entry vector for the human APN gene.

Depositor

Sho Iketani, Alejandro Chavez

Insert

APN (ANPEP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-RNASEL_IDG-K

Plasmid

#170604

Purpose

Gateway destination clone of RNASEL (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-RNASEL (RNASEL Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Grc3

Plasmid

#63760

Purpose

yeast ORF Grc3 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Grc3

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.HRAS

Plasmid

#83181

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NIM1K_IDG-K

Plasmid

#189892

Purpose

Gateway destination clone of NIM1K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NIM1K (NIM1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

Plasmid

#158160

Purpose

Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-3xHA-miniTurbo-3XNLS

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Hs.HRAS G12D

Plasmid

#83183

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12D

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221-FURIN

Plasmid

#158442

Purpose

Gateway Cloning compatible entry vector for the human FURIN gene.

Depositor

Sho Iketani, Alejandro Chavez

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Flag-TLK2_IDG-K

Plasmid

#135278

Purpose

Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

Depositor

Ben Major

Insert

Flag-TLK2 (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-CTSB

Plasmid

#158443

Purpose

Gateway Cloning compatible entry vector for the human CTSB gene.

Depositor

Sho Iketani, Alejandro Chavez

Insert

CTSB (CTSB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDESTsmg

Plasmid

#18878

Depositor

Ted Baker

Article

Goldstone et al Protein Expr Purif. 2008 Jan . 57(1):81-

Type

Empty backbone

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PKMYT1-V5-miniTurbo_IDG-K

Plasmid

#135246

Purpose

Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PKMYT1-V5-miniTurbo (PKMYT1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PIP5K1B_IDG-K

Plasmid

#170599

Purpose

Gateway destination clone of PIP5K1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PIP5K1B (PIP5K1B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-TLK2-V5_IDG-K

Plasmid

#135251

Purpose

Gateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TLK2-V5 (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R777-E061 Hs.ERBB2

Plasmid

#70345

Purpose

Gateway ORF clone of human ERBB2 [NM_004448.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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