Addgene

Showing: 1201 - 1220 of 5232 results

Results per page:

Sort results by:

pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
medSIIN

Plasmid

#185664

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hiSIIN

Plasmid

#185662

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
dimSIIN

Plasmid

#185661

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
loSIIN

Plasmid

#185663

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
DT034A

Plasmid

#159395

Purpose

Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt

Use

Synthetic Biology

Tags

FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

Expression

Bacterial

Mutation

Promoter

J23117 and pLux promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DT050A

Plasmid

#159411

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

J23101 promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CS51

Plasmid

#117433

Purpose

Expression of ARL3

Depositor

Richard Kahn

Article

Cavenagh et al J Biol Chem. 1994 Jul 22;269(29):18937-4

Insert

ARL3 (ARL3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo Diap3 in situ probe

Plasmid

#45602

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Diap3 in situ probe (Diaph3 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 1589-2321 of BC028920

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Crim1 in situ probe

Plasmid

#45600

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crim1 in situ probe (Crim1 Mouse)

Use

In situ

Tags

Expression

Mutation

insert contains bp#4062-4502 of NM_015800.3

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DT049A

Plasmid

#159410

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tet promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge