pb-NES-ZapCV5 (cpV143)
Plasmid
#112061
Purpose
Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
Depositor
Insert
NES-ZapCV5
Use
Tags
Nuclear Export Signal (NES) derived from HIV-1
Expression
Mammalian
Mutation
ECFP gene is truncated 36 bp at 3' end. Venu…
Promoter
CBA
Available since
July 9, 2018
Availability
Academic Institutions and Nonprofits only