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Plasmid#162933PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoterDepositorInsertHis6-maltose binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT048A
Plasmid#159409PurposeConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23101-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromoterJ23101 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT046A
Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide
Plasmid#121993PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide#2
Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-opa1 guide
Plasmid#121995PurposeAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expressionDepositorInsertopa1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domaiā¦PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only