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Plasmid#224545PurposeA Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyADepositorInsertWPRE-SV40-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB113
Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
UseTags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS N terminal HA
Plasmid#217639PurposeEntry vector with WT1 +KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS C terminal HA
Plasmid#217640PurposeEntry vector with WT1 +KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS N terminal HA
Plasmid#217641PurposeEntry vector with WT1 -KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS C terminal HA
Plasmid#217642PurposeEntry vector with WT1 -KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 genomic A. thaliana PLDGAMMA3
Plasmid#209188PurposepENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tagsDepositorInsertAT4G11840 (PLDGAMMA3 Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only