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Addgene
Showing: 11981 - 12000 of 16040 results

  1. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    John Ngo
    Article
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Insert
    dCas9-NS3-NLS/VPR
    Use
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    112244-plasmid-map-sequence-id-216918

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  2. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  4. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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  5. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

    Plasmid
    #169549
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Availability
    Academic Institutions and Nonprofits only
    169549-plasmid-map-sequence-id-364990

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  6. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid
    #169550
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Availability
    Academic Institutions and Nonprofits only
    169550-plasmid-map-sequence-id-366597

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  10. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

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Showing: 11981 - 12000 of 16040 results