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Plasmid#195333Purposevector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized xCas12i
UseTags3xFlagExpressionMammalianMutationPromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1
Plasmid#155346PurposeExpress full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF1 (YTHDF1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
ShuttleNSE
Plasmid#50958PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterNSEAvailable sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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CFP-Parkin
Plasmid#47560PurposeCFP tagged Parkin for colocalization studyDepositorInsertParkin (PRKN Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
MBD2a pcDNA3.1
Plasmid#78141PurposeMammalian expression of MBD2aDepositorInsertMBD2a (MBD2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF3
Plasmid#155348PurposeExpress full-length human YTHDF3 with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF3 (YTHDF3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMADM-beta
Plasmid#36891DepositorInsertsBeta-Geo
thymidine kinase
UseTagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF2
Plasmid#155347PurposeExpress full-length human YTHDF2 with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARα.hRXRα
Plasmid#135411PurposeHuman Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-alpha
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 C506S
Plasmid#78584PurposeExpression flag-USP19 C506S in mammalian cellsDepositorInsertflag-USP19 C506S (USP19 Human)
UseTagsflagExpressionMammalianMutationmutate 506 cysteine residue to serinePromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab7Q67L
Plasmid#169038PurposeExpress human constitutively active form of Rab7a(Q67L) in mammalian cellsDepositorInsertRab7a (RAB7A Human)
UseTagsacGFP1ExpressionMammalianMutationQ67LPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
vsv-Sgo1-GFP
Plasmid#108494Purposeexpression of vsv-Sgo1-EGFPDepositorInsertShugoshin 1 (SGO1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin
Plasmid#141308PurposeMammalian expression of FLAG-tagged TapasinDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARγ.hRXRα
Plasmid#135416PurposeHuman Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-gamma
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRARβ.hRXRα
Plasmid#135415PurposeHuman Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vectorDepositorInsertsHuman retinoic acid receptor-beta
Human retinoid X receptor-alpha
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBD2b pcDNA3.1
Plasmid#78142PurposeMammalian expression of MBD2bDepositorInsertMBD2b (MBD2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-GGA3-GAT
Plasmid#192259PurposeMammalian expression of GFP-tagged GGA3-GATDepositorInsertGGA3-GAT (GGA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Myo1b
Plasmid#187366PurposeExpresses rat myosin 1b isoform b domain labelled with CherryDepositorInsertmyosin 1b isoform b (Myo1b Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only