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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mutation

see attached file

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23117

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1282-1792

Plasmid

#40960

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1281

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Article

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABE8eHD-BlastR

Plasmid

#176478

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABE8eHD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo

Plasmid

#179393

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_EGFP

Plasmid

#179392

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

EGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC626

Plasmid

#174367

Purpose

Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23106

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-EGFP

Plasmid

#196609

Purpose

siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + EGFP inverted repeat sequence

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

AraC

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_2xChromo

Plasmid

#179398

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Alx3

Plasmid

#80382

Purpose

mammalian expression of rat Alx3

Depositor

Mario Vallejo

Article

Perez-Villamil et al J Biol Chem. 2004 Sep 3;279(36):38062-71

Insert

Alx3 (Alx3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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