EGFP-bSV1_1-1009
(Plasmid #129471)

• Purpose: Mammalian expression of the N-terminus + central domain of bovine SV1
• Depositing Lab: Elizabeth Luna
• Publication: Wulfkuhle et al J Cell Sci. 1999 Jul . 112 ( Pt 13)():2125-36.

Backbone

• Vector backbone: pEGFPC
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Supervillin
• Alt name: SVIL
• Alt name: Supervilin, isoform 1
• Species: B. taurus (bovine)
• Mutation: contains amino acids 1-1009
• Entrez Gene: SVIL
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please not there are multiple predicted isoforms of supervillin [Bos taurus]. The sequence is this plasmid also aligns to isoform supervillin X20