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Addgene
Showing: 101 - 120 of 27750 results

  1. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    134842-plasmid-map-sequence-id-266572

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  2. pAK_DR30_CasRx_Puro

    Plasmid
    #134845
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    134845-plasmid-map-sequence-id-266706

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  4. pLENTI_hACE2_PURO

    Plasmid
    #155295
    Purpose
    Lentiviral vector to generate hACE2 stable expressing cell line
    Depositor
    Raffaele De Francesco
    Insert
    hACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    C9
    Expression
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    155295-plasmid-map-sequence-id-307855

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  6. pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

    Plasmid
    #163755
    Purpose
    piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes
    Depositor
    Kurt Pfannkuche
    Article
    Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1
    Insert
    mCherry
    Use
    Piccybac transposon
    Tags
    puromycin-N-acetyltransferase
    Expression
    Mammalian
    Mutation
    Promoter
    human NCX1
    Availability
    Academic Institutions and Nonprofits only
    163755-plasmid-map-sequence-id-326668

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  7. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  8. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  18. pAK_Tol2_TRE_Puro

    Plasmid
    #130259
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Availability
    Academic Institutions and Nonprofits only
    130259-plasmid-map-sequence-id-255016

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Showing: 101 - 120 of 27750 results