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pEBTet-SNAP-Cep78

Plasmid

#136827

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep78 (CEP78 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

Plasmid

#111903

Purpose

Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

Depositor

Andrew Carter

Inserts

His-ZZ-LTLT-SNAPf-DYNC1H1
DYNC1I2
DYNC1LI2
DYNLT1
DYNLRB1
DYNLL1

Use

Tags

8xHis, SNAPf-tag, TEV site, and ZZ-tag

Expression

Insect

Mutation

Optimised for expression in Sf9 cells.

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-Snapin

Plasmid

#118742

Purpose

Expresses EGFP tagged Snapin

Depositor

Beverly Koller

Insert

Snapin cDNA (Snapin Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICDR1

Plasmid

#111858

Purpose

N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

R250Q, Optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-SNAP-TM

Plasmid

#207319

Purpose

Functional incorporation of SNAP-tag into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SNAP-tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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58 pLIB_SNAP

Plasmid

#160307

Purpose

N-term 3C cleavable SNAP tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

12xHis and SNAP

Expression

Insect

Mutation

Promoter

polyhydrin

Availability

Academic Institutions and Nonprofits only

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UAS-myr::4xSNAPf

Plasmid

#87637

Purpose

For creating drosophila transgenics expressing UAS-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xSNAPf

Plasmid

#87638

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-SNAP

Plasmid

#195164

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with SNAP tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSNAP-CaaX Control Plasmid

Plasmid

#101133

Purpose

Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

Depositor

Ana Egana, New England Biolabs

Insert

CaaX Box

Use

Tags

SNAP-tag (SNAP26m)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_SNAP-p24

Plasmid

#130718

Purpose

Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-p24

Use

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pSNAPf-H2B Control Plasmid

Plasmid

#101124

Purpose

Expression of SNAP-tagged Histone H2B in mammalian cells

Depositor

Ana Egana, New England Biolabs

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Use

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-SNAPf-WPRE

Plasmid

#104106

Purpose

Expresses SNAPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-ADRβ2 Control Plasmid

Plasmid

#101123

Purpose

Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

Depositor

Ana Egana, New England Biolabs

Insert

Beta-2-Adrenergic Receptor (ADRB2 Human)

Use

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge