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pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170813

Purpose

Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

PH-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_iRFP_P2AT2A_mScarlet-I_Myl9

Plasmid

#172474

Purpose

Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

Depositor

Sean R Collins

Article

Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

Inserts

iRFP670
mScarlet-I-Myl9 (MYL9 Human)

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

EF1alpha (with UCOE) and none (same transcript as…

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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3_T7-mScarlet3-KRas6R

Plasmid

#225930

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-KRas6R

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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1_T7-mScarlet3-Hras

Plasmid

#225928

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-HRas

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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2_T7-mScarlet3-KRas

Plasmid

#225929

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-KRas

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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4_T7-mScarlet3-RitC

Plasmid

#225931

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-RitC

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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5_T7-GAP43-mScarlet3

Plasmid

#225932

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

GAP43-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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6_T7-Lyn-mScarlet3

Plasmid

#225933

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

Lyn-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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7_T7-Src64B-mScarlet3

Plasmid

#225934

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

Src64B-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-LactC2

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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10_T7-SP-CD8tm-mScarlet3

Plasmid

#225937

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-CD8tm-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mScarlet3

Plasmid

#219640

Purpose

Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mScarlet SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_64 [mScarlet]

Plasmid

#198975

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

mScarlet-I

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_C1

Plasmid

#85044

Purpose

Mammalian expression of mScarlet-i

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet-i

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LMNA_mScarlet-I_Donor

Plasmid

#178092

Purpose

HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA-mScarlet-I HDR donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I_Donor

Plasmid

#173207

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170816

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170815

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pVY-11 mScarletI-tag3

Plasmid

#194626

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

mScarlet-I-(GGSKKRKKR)3

Use

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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