-
Plasmid#75030PurposeRetroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)DepositorInsertUbiquitin
UseRetroviralTagsHA and IRES-eGFPExpressionMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMX-HA-UbvG08_MUT-IRES-GFP
Plasmid#75031PurposeRetroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)DepositorInsertUbiquitin
UseRetroviralTagsHA and IRES-eGFPExpressionMutationUbvG08 with I44A, P69L, and L70V mutations and no…PromoterAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-mNINJ1-GFP
Plasmid#208776PurposeMSCV vector expressing WT mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPExpressionMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT GLUT1-GFP
Plasmid#200093PurposeWT human GLUT1 with a C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
1K245-->R GLUT1-GFP
Plasmid#200095Purpose1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK245RPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato-2AcytoGFP
Plasmid#53124PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; Expression in chicken cellsTags2A-cyto-GFP and memTdTomatoExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP
Plasmid#188643PurposeThis is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genesDepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-K45Q-GFP
Plasmid#208777PurposeMSCV vector expressing K45Q mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPExpressionMutationChange Lys 45 to Gln (K45Q)PromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-D53A-GFP
Plasmid#208778PurposeMSCV vector expressing D53A mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPExpressionMutationChanged aspartic acid 53 to alanine (D53A)PromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLIP-puro-dsRed-GFP-miRNA
Plasmid#32704DepositorInsertemGFP-miRNA
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-CDMPR
Plasmid#182642PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsEGFP and N-terminal ER targeting sequence of hema…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXsIP GFP-VPS33A
Plasmid#67022Purposehuman VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vectorDepositorInsertVacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP VPS16-GFP
Plasmid#67023PurposeHuman VPS16 ORF was inserted into pMRXIP GFP-N vectorDepositorInsertHomo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
6K-loop-->R GLUT1-GFP
Plasmid#200098Purposehuman GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 225, 229, 230, …PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
5K-tails-->R GLUT1-GFP
Plasmid#200099Purposehuman GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 451, 456,…PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
1K245 GLUT1-GFP
Plasmid#200097Purposehuman GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 225, 229,…PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
11K-cyto-->R GLUT1-GFP
Plasmid#200096Purposehuman GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 225, 229,…PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only