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Plasmid#220446PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Cas9-hGem_Smc4
Plasmid#217667Purposeexpresses Cas9-hGem and guideRNA for tagging of SMC4DepositorInsertsgRNA1 targeting SMC4 C-terminal (SMC4 Human)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(2)
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-ExSYTE
Plasmid#216265PurposeExSYTE in oocyte vectorDepositorInsertExSYTE
UseCrna synthesis for xenopus laevis oocyteTagsnoneExpressionMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G137I)
Plasmid#133272PurposeX. laevis expression vector. It will generate the mouse TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseOocyte expressionTagsExpressionMutationG137IPromoterT7Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
etnppl-pGEMtEasy
Plasmid#192263PurposeMouse Etnppl sequence is inserted in pGEM-T Easy vector.DepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1_cryst
Plasmid#133271PurposeX. laevis expression vector. It will generate the TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
UseOocyte expressionTagsExpressionMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…PromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM3/cGata1
Plasmid#83829PurposeCloning of chicken GATA1DepositorInsertGata1 (GATA1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxb4a
Plasmid#27523DepositorInsertHoxb4a (hoxb4a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxc10a
Plasmid#27536DepositorInsertHoxc10a (hoxc10a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxd10a
Plasmid#27543DepositorInsertHoxd10a (hoxd10a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxd11a
Plasmid#27544DepositorInsertHoxd11a (hoxd11a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxc8a
Plasmid#27538DepositorInsertHoxc8a (hoxc8a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Clover-Geminin(1-110)
Plasmid#83915PurposeFluorescent probe for M/G1 transitionDepositorInsertClover-Geminin(1-110)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEM-GECO1
Plasmid#32442PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO1
UseTagsExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21
Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only