Skip to main content
Addgene
Showing: 101 - 120 of 4743 results

  1. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

    Enlarge

  2. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

    Enlarge

  3. pEGPN-coBCL2L1-G94A

    Plasmid
    #188697
    Purpose
    express codon optimized BCL-XL drug resistant mutant
    Depositor
    Lewis Cantley
    Insert
    coBCL2L1-G94A (BCL2L1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    codon optimized, G94A
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188697-plasmid-map-sequence-id-375004

    Enlarge

  4. pEGPN-coBCL2L1-F97C

    Plasmid
    #188696
    Purpose
    express codon optimized BCL-XL drug resistant mutant
    Depositor
    Lewis Cantley
    Insert
    coBCL2L1-F97C (BCL2L1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    codon optimized, F97C
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188696-plasmid-map-sequence-id-374883

    Enlarge

  5. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

    Enlarge

  10. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

    Enlarge

  12. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Joe Landry
    Insert
    Human H2AZ.1 (H2AZ1 Synthetic, Codon Optimized, Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42629-plasmid-map-sequence-id-434040

    Enlarge

  13. pGEX6P1 METTL3

    Plasmid
    #200731
    Purpose
    Bacterial expression of GST-Tagged codon optimized human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200731-plasmid-map-sequence-id-401808

    Enlarge

  14. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Keith Joung
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Use
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    140083-plasmid-map-sequence-id-292705

    Enlarge

  15. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40786-plasmid-map-sequence-id-68901

    Enlarge

  16. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40787-plasmid-map-sequence-id-68902

    Enlarge

  17. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-198}:NosT

    Plasmid
    #212166
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212166-plasmid-map-sequence-id-422670

    Enlarge

  18. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-216}:NosT

    Plasmid
    #212167
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (216bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212167-plasmid-map-sequence-id-422675

    Enlarge

  19. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-390}:NosT

    Plasmid
    #212168
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (390bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-390}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212168-plasmid-map-sequence-id-422679

    Enlarge

Showing: 101 - 120 of 4743 results