-
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only
-
Str-KDEL_flGalT-SBP-EGFP
Plasmid#65272Purposesynchronize trafficking of GalT from the ER (RUSH system)DepositorInsertgalactosidase
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_flGalT-SBP-mCherry
Plasmid#65273Purposesynchronize trafficking of GalT from the ER (RUSH system)DepositorInsertgalactosidase
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SBP-EGFP-Ecadherin_puromycin
Plasmid#65292PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
ii-Str_SBP-mCherry-GPI
Plasmid#65297Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SBP-mCherry-Ecadherin_puromycin
Plasmid#65293PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_flGalT-SBP-tagBFP
Plasmid#65274Purposesynchronize trafficking of GalT from the ER (RUSH system)DepositorInsertgalactosidase
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-SGTAP no-pA
Plasmid#82599Purpose3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purificationDepositorInsertSBP-TEV-2x protein G
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
flGalT-SBP-EGFP_puromycin
Plasmid#65275PurposeGalT reporter only ( no hook) (RUSH system)DepositorInsertgalactosidase
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
flGalT-SBP-mCherry_puromycin
Plasmid#65276PurposeGalT reporter only ( no hook) (RUSH system)DepositorInsertgalactosidase
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-Strep(II)
Plasmid#72984PurposeStreptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertStrep(II) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-GPI
Plasmid#65298Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequenceExpressionMutationPromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-mCherry
Plasmid#65279Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_TNF-SBP-EGFP
Plasmid#65280Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-EGFP
Plasmid#65278Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-GPI
Plasmid#65299Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-RpHLuorin2
Plasmid#171722PurposeSynchronize trafficking of TNFa-RpHLuorin2DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and ratiometri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
UseTagsE6 tagExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only