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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ChrimsonR-tdTomato

Plasmid

#99231

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CaMKII

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chrimson-GFP

Plasmid

#62718

Purpose

AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP-p2a-ChrimsonR

Plasmid

#178794

Purpose

Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and ChrimsonR

Use

Tags

mRuby2

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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13XLexAop2 IVS ChrimsonR mCherry_tr

Plasmid

#111548

Purpose

Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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H517 SONIC HRASV12 donor

Plasmid

#138177

Purpose

CRISPR SONIC: HRAS G12V donor plasmid

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

HRASV12 (HRAS Human)

Use

Nhej donor

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-ICP8 (pSLIK1)

Plasmid

#113856

Purpose

For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

Plasmid

#183803

Purpose

AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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ZE10 sonic hedgehog pT7TS

Plasmid

#17059

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

sonic hedgehog (shha Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
PX383 Lactobacillus johnsonii Cas9

Plasmid

#68320

Purpose

Lactobacillus johnsonii Cas9

Depositor

Feng Zhang

Insert

LjCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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ZE12 sonic hedgehog CMV

Plasmid

#17061

Depositor

Randall Moon

Insert

sonic hedgehog (shha Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-PAG-E2-Crimson-3XNLS-pA

Plasmid

#183801

Purpose

AAV vector to express nuclear localized E2-Crimson from PAG promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

Plasmid

#102770

Purpose

"Activate neurons with 2p stimulation"

Depositor

Karel Svoboda

Article

Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

Insert

ChrimsonR::FusionRed::Kv2.1

Use

AAV

Tags

FusionRed and Kv2.1 traficking domain

Expression

Mutation

Promoter

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

Plasmid

#62723

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV1 and AAV5

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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