Purpose
Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
Inserts
Chloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
Expression
Bacterial
Mutation
111TAG and 8TAG/112TAG
Promoter
AraC, T7, cat promoter (constitutive), and lpp (c…
Available since
April 10, 2023
Availability
Academic Institutions and Nonprofits only