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Showing: 101 - 120 of 130 results

  1. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

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  2. pIA036

    Plasmid
    #200425
    Purpose
    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    200425-plasmid-map-sequence-id-443100

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  6. pcDNA3-N-I-N-CAAX

    Plasmid
    #41686
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    Use
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41686-plasmid-map-sequence-id-55893

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  7. pcDNA3-mNeptune2-N

    Plasmid
    #41645
    Purpose
    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    mNeptune2-Dronpa145N
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K145N on Dronpa (allows control of photochromic F…
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41645-plasmid-map-sequence-id-296256

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  8. pcDNA3-K-I-N-CAAX

    Plasmid
    #41640
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    Use
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41640-plasmid-map-sequence-id-240702

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  17. FLINC-AKAR1-CAAX

    Plasmid
    #87705
    Purpose
    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1-CAAX
    Use
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87705-plasmid-map-sequence-id-176711

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  18. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  19. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  20. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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Showing: 101 - 120 of 130 results