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Plasmid#74056Purposeretroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion proteinDepositorInsertBirA biotin ligase
UseRetroviralTagsP2A-mCherryExpressionMammalianMutationPromoterMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 IGF1R-BirA(R118G)-HA
Plasmid#109232PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).DepositorInsertIGF1R (IGF1R Human)
UseTagsBirA(R118G) (highly promiscuous form) and HAExpressionMammalianMutationPromoterT7Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/TO-myc-BirA*-NES
Plasmid#112714PurposeFor tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.DepositorInsertHIV Rev nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
BirA(R118G)-HA destination vector
Plasmid#53581PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)DepositorTypeEmpty backboneUseTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-FLAG-BirA-TEV
Plasmid#105770PurposeExpression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsFLAG-BirA-TEVExpressionMammalianMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTags6-Histidine-tagExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA
Plasmid#113896PurposeMammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd:CMV-rtTA-IRES-birA
Plasmid#31375DepositorInsertrtTA-IRES-BirA
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-NLS-BirA-2A-mCherry
Plasmid#127781PurposeBirA construct in a PCI nls backbone driving mCherry activityDepositorInsertBirA
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorInsertsUseTagsGFPExpressionWormMutationPromoterhis-72 and unc-119Available sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA-ER
Plasmid#113897PurposeMammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-BirA*-KRASG12V
Plasmid#122208PurposeExpresses myc-BirA* fused to KRASG12V in mammalian cellsDepositorInsertKRASG12V (KRAS Human)
UseTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/FKBP12-pBirA(284-C)-HA
Plasmid#154452Purposeexpresses FKBP12-pBirA(284-C)-HA/biochemical assayDepositorInsertFKBP12-pBirA(284-C)-HA (FKBP12 Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only