Addgene

We narrowed to 2,025 results for: CO

Showing: 251 - 300 of 2025 results

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pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mTRQ2 and mVenus

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-NN

Plasmid

#105125

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mTRQ2/mVenus

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CC

Plasmid

#105116

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mTRQ2/mVenus

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-CC

Plasmid

#105115

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mEGFP/mCherry

Expression

Plant

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETtv-2in1-NC

Plasmid

#105122

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mTRQ2 and mVenus

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETgc-2in1-NC

Plasmid

#105121

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mCherry and mEGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

Plasmid

#216382

Purpose

Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

6xHis-3C-splitGFP11

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis-p47

Plasmid

#21268

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

p47 (Nsfl1c Rat)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-CN

Plasmid

#105118

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mCherry and mEGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-Ufd1-His

Plasmid

#21266

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

Ufd1 (Ufd1l Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETgc-2in1-NN

Plasmid

#105124

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mEGFP/mCherry

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyr_M37_KDEL

Plasmid

#51530

Purpose

Ectopic expression of Lyr with KDEL tag for CTAP

Depositor

Claus Jorgensen

Article

Tape et al Mol Cell Proteomics. 2014 May 12.

Insert

Lysine Racemase

Use

Tags

HA and KDEL

Expression

Mammalian

Mutation

aa's 1-36 removed

Promoter

CMV

Available since

April 29, 2014

Availability

Academic Institutions and Nonprofits only
pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B1979

Plasmid

#59181

Purpose

Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

Blasticidin S resistance

Use

Tags

Expression

Worm

Mutation

Promoter

rpl-28

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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p817-mgammaFcry-EGFP

Plasmid

#23156

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:EGFP (eGFP mouse gammaF promoter:EGFP, Mouse)

Use

Multicloning site flanked by i-scei sites

Tags

Expression

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Promoter

Available since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV_S

Plasmid

#100266

Purpose

pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli

Depositor

Timothy Lu

Article

Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.

Inserts

entD (entD Escherichia coli)
entC
entE
entB
entA
vibH
vibF
cos

Use

Synthetic Biology; Expression, pbr322

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETvr-2in1-NC

Plasmid

#105123

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus and tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETvr-2in1-CN

Plasmid

#105120

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus and tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ4-(HA)3

Plasmid

#198180

Purpose

Expression of HA-tagged AP-4 μ4 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-4 μ4

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p817-mgammaFcry-mCherry

Plasmid

#23154

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:mCherry (Crygf Mouse)

Use

Multicloning site flanked by i-scei sites

Tags

mCherry

Expression

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Promoter

Available since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
JC101

Plasmid

#104952

Purpose

Methanol induing RFP, estrogen inducing GFP, and IPTG inducing CFP

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

GFP, mCherry, and CFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-3xflagGREB1c

Plasmid

#112223

Purpose

mammalian expression of flag tagged GREB1c

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1c

Use

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETvr-2in1-NN

Plasmid

#105126

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JC172

Plasmid

#104948

Purpose

Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA-hGH fusion protein and TEV protease

Use

Tags

Expression

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JC031

Plasmid

#104953

Purpose

Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing Interferon

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

hGH, Interferon and G-CSF

Use

Tags

Expression

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ZE37 Nlk1(K117M)-pCS2+

Plasmid

#17078

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

K117M. Kinase dead mutant of NLK1.

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE36 Nlk1(C387Y)-pCS2+

Plasmid

#17077

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

C387Y. Inactive mutant of NLK1.

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pP4-C

Plasmid

#225097

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Article

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Use

Tags

Expression

Bacterial

Mutation

Promoter

synthetic constitutive promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLC25A13-APEX2

Plasmid

#228198

Purpose

plasmid encoding human SLC25A13 with an APEX protein tag

Depositor

Stephen Benkovic

Article

Sha et al J Biol Chem. 2024 Aug 2;300(9):107620. d

Insert

SLC25A13 (SLC25A13 Human)

Use

Synthetic Biology

Tags

APEX2

Expression

Mutation

Promoter

M13

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB5230

Plasmid

#215496

Purpose

A pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

Pil1-mCherry-SpAtg8(1-115)

Use

Tags

Expression

Yeast

Mutation

Promoter

P41nmt1

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-MCS1-GB1_AB

Plasmid

#148370

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-MCS2-GB1_AB

Plasmid

#148371

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP002

Plasmid

#103872

Purpose

Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Inserts

hph
Spcas9 D147Y P411T

Use

CRISPR

Tags

NLS

Expression

Yeast

Mutation

D147Y P411T

Promoter

Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCold CL7-Cas9

Plasmid

#124890

Purpose

obtaining full Cas9 RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF806-reci

Plasmid

#186711

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPglu-600u

Plasmid

#13572

Depositor

Wolf Frommer

Article

Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

Insert

FLIPglu F16A

Use

Tags

ECFP and EYFP

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
peGFP-N1-SHB

Plasmid

#139605

Purpose

Expresses eGFP-SHB fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

SHB (SHB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD238-1a

Plasmid

#90958

Purpose

Expresses S. baltica TtrS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23109

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-hPIASx beta

Plasmid

#15210

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx beta (PIAS2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCold CL7-Cas12a

Plasmid

#124891

Purpose

obtaining full Cas12a RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas12a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C'5802

Plasmid

#176879

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUGI-NLS

Plasmid

#101091

Purpose

Expresses UGI in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 251 - 300 of 2025 results

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