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Plasmid#146647PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutationG26S relative to NM_166265.2/NP_725750.1PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D CA
Plasmid#52247PurposeDrosophila expression of YFP-Ras85D constitutively active mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc-mCh-Tub
Plasmid#24288DepositorInsertAlpha-Tubulin (alphaTub84B Fly)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D WT
Plasmid#52242PurposeDrosophila expression of YFP-Ras85DDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
AByG
Plasmid#111083PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInserts3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B
Plasmid#21682DepositorInsertmaternal expression at 31B (me31B Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmG3BP_G
Plasmid#146349PurposeInsect Expression of DmG3BPDepositorInsertDmG3BP (rin Fly)
UseTagsExpressionInsectMutationV632G compared to AF231031.1, exact match to NP_5…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed-w+
Plasmid#80801PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.DepositorInsertspiggyBac-3xP3-DsRed
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D DN
Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B DN
Plasmid#52243PurposeDrosophila expression of YFP-Ras64B dominant negative mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS19N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B CA
Plasmid#52244PurposeDrosophila expression of YFP-Ras64B constitutively active mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ63L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B WT
Plasmid#52245PurposeDrosophila expression of YFP-Ras64BDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAS-syb:spGFP1–10
Plasmid#73698PurposeNeuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spGFP1–10 chimera
UseDrosophilaTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS6 AA 1035-1284
Plasmid#196904PurposeExpresses His-tagged Drosophila IntS6 amino acids 1035-1284DepositorInsertIntegrator subunit 6 (IntS6 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
Plasmid#79259PurposeExpresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cellsDepositorInsertDmeIF4G (eIF4G1 Fly)
UseSchneider 2 cell expressionTagsV5-His6 and lambdaN-HAExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
UseTagsExpressionInsectMutation322-1666 truncated version of elF4G sequence with…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRTagsExpressionMutationPromoterU6:1, U6:3, and dU6-1; dU6-3Available sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD2-dU6:2gRNA
Plasmid#49409Purposeexpression of gRNA under control of the Drosophila U6:2 promoterDepositorInsertdU6-2:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-2Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only