-
Plasmid#166136PurposeGateway entry vector for mRuby3DepositorArticleInsertmRuby3
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxamEXP4CUP1
Plasmid#218284PurposeUse together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTHmut-EGFP
Plasmid#209323PurposeAAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1--YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC025 - pL0_StopCodon (CT)
Plasmid#203905PurposeMoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.DepositorInsertStop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)
Plasmid#214878PurposeLentiviral vector encoding RfxCas13d targeting SURF1 guide arrayDepositorInserthU6-crSURF1-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)
Plasmid#214882PurposeLentiviral vector encoding RfxCas13d targeting PROX guide arrayDepositorInserthU6-crPROX-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Myc-XIAP
Plasmid#217627PurposeMammalian expression of Myc tagged XIAPDepositorInsertXIAP (XIAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A-E1021A
Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A-E1021A
Plasmid#213414PurposeVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEWS
Plasmid#208799PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381A; S437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-3A
Plasmid#208800PurposeXenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationV473A; R476A; Y481APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-Δ24
Plasmid#208801PurposeXenopus laevis His6-Myc-DONSON (1-24aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationΔ24, deletion of 1-24aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEW
Plasmid#208802PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry
Plasmid#202090PurposeNeuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutationsDepositorInsertdCas9-Set2(R195G, C201A)
UseTags3x FLAG and mCherryExpressionMammalianMutationR195G and C201A abolish catalytic activityPromoterhuman SynapsinAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-Δ220-234-Flag
Plasmid#210341PurposeExpresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationΔ220-234PromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-H120N/F121L-Flag
Plasmid#210340PurposeExpresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationH120N/F121LPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-FR-2Strep
Plasmid#210316PurposeExpresses SARS-CoV-2 NSP4 in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-mCherry
Plasmid#210319PurposeExpresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsmCherryExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT-CNP
Plasmid#211803PurposeExpresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domainDepositorInsert2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
UseTagsExpressionBacterialMutationPromotertetAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-GEMS(JAK-STAT)
Plasmid#209109PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)DepositorInsertA-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only