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Plasmid#159889PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-2(no_start)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1F
Plasmid#135660PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-E2A-cyOFP1
Plasmid#141084Purposeprecursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-E2A-cyOFP1
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP89b CvTA V124N
Plasmid#141207PurposeChromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationV124NPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-SBS-mCherry
Plasmid#134598PurposeLow Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)DepositorInsertSBS-mCherry
UseTagsmCherryExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-T7-CBD-hGLY
Plasmid#134940PurposeT7 based expression of human glycine acyltransferase with an N-terminal chitin binding domainDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
UseTagschitin binding domainExpressionBacterialMutationPromotert7Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
UseTagsExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-pT7-sfGFP_RED20
Plasmid#135173PurposeExpresses sfGFP recoded in the RED20 genetic code off the T7 promoterDepositorInsertsfGFP_RED20
UseSynthetic BiologyTagsExpressionMutationRecoded ORF of sfGFP to only use one codon per am…PromotertaatacgactcactataAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet139
Plasmid#121913PurposeCharge-Introduced NpuDnaB mini-inteinDepositorInsertCI-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet140
Plasmid#121914PurposeOrthogonal NpuDnaB mini-intein inteinDepositorInsertOth-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtCRY2-PHR_X
Plasmid#131180PurposeExpresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtCRY2-PHR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)
Plasmid#132637Purposeexpresses mouse Tankyrase1 ARC2-3 (308-655) in E. coliDepositorInsertTankyrase1 (308-655)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.178
Plasmid#119645PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS3 Down Flank
UseSynthetic BiologyTagsExpressionMutation4 nucleotide insertion between nt 65 and 66 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.165
Plasmid#119634PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS1 Up Flank
UseSynthetic BiologyTagsExpressionMutation1 nucleotide insertion between nt 21 and 22 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_EcSSPB-micro
Plasmid#131218PurposeExpresses EcSSPB-nano (Escherichia coli) preceded by restriction sitesDepositorInsertEcSSPB-micro
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_EcSSPB-nano
Plasmid#131219PurposeExpresses EcSSPB-micro (Escherichia coli) preceded by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-micro_X
Plasmid#131213PurposeExpresses EcSSPB-micro (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-micro
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only