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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

UAS

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Insert

hM4D-2a-GFP (CHRM4 Human, Synthetic)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

Plasmid

#208696

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

Plasmid

#58959

Purpose

lentiviral expression of v-H-Ras and luciferase/EGFP reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer/chicken beta actin

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP-B19G

Plasmid

#52473

Purpose

Expresses splitTVA-EGFP-B19G

Depositor

Ian Wickersham

Article

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Has Service

AAV1

Insert

TVA-P2A-EGFP-P2A-B19G

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

Plasmid

#24378

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::p150yeGFP-DHFR

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

Plasmid

#70741

Purpose

1 color strong reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 Y637S-IRES-GFP

Plasmid

#91887

Purpose

Retroviral expression of mouse Ezh2 with Y637S

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Y637S

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-TLR4 delta tail

Plasmid

#133063

Purpose

Retroviral expression of mouse TLR4 with out its cytosolic tail

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

TLR4 delta tail (Tlr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

Plasmid

#75115

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

deleted bromodomain

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Jean Zhao, Kira Gritsman

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Use

Tags

flag and myristoylation tag

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA

Plasmid

#168101

Purpose

GFP and Flag tagged Nup62 with EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

GFP-Flag-Nup62-EPEA

Use

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)

Plasmid

#70742

Purpose

1 color weak reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

Plasmid

#134869

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome b5 type A (CYB5A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-98

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

Plasmid

#51656

Purpose

hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

Depositor

Adam Cohen

Insert

Quasar2-mOrange-Chr64-GFP

Use

Lentiviral

Tags

GFP and mOrange

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

Plasmid

#129111

Purpose

Homologous recombination vector for excision reporter 1 in the CLYBL locus.

Depositor

Bruce Conklin

Insert

LSL, eGFP, NLS-RFP

Use

Cre/Lox and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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