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Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_A2S-8
Plasmid#188654PurposeAAV vector expressing SaAID-2S and gRNADepositorInsertsAID2S--nSaCas9-UGI
Sa-sgRNA
UseAAV and CRISPRTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterScp1 and U6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAT-lux
Plasmid#20890DepositorInsert7xTCF binding sites, Siamois minimal promoter
UseLuciferaseTagsExpressionMammalianMutation7 multimerized TCF-binding elements fused upstrea…PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
E2_pENTR_R4R3_T CTP
Plasmid#171736PurposeptpTALECD vector assembly, Entry clone 2 with attR4 and R3DepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-TIAM1 DH/PH
Plasmid#81129Purposemammalian expression of Zdk1 tagged Tiam1 Dh/PHDepositorInsertsUseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
IDP424
Plasmid#87018Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SP
Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS1-gfp
Plasmid#109288PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertMultiple Cloning Site and GFP
UseTagsExpressionBacterialMutationPromoterKanamycinAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BAP-Sox2
Plasmid#133281PurposeExpression of target proteins BAP-Sox2 in mammalian cellsDepositorInsertSox2 (SOX2 Human)
UseTagsBiotin Acceptor Peptide contains 7-His-tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL1079
Plasmid#116897PurposeCenpC:eGFP (centromere marker)DepositorInsertsCenp C (partial)
eGFP
CenpC downstream region
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationtruncated, no stop codon and without start codonPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet
Plasmid#85577PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-.008
Plasmid#140140PurposeEvaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-PhCHS-CmCHI
Plasmid#73401PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
JZ901: pMVP (L5-L4) EF1a promoter
Plasmid#121727PurposepMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Plasmid#128167PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)DepositorInsertmTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p alpha-actinin:GAL4/UAS:TdTomato
Plasmid#161712PurposeExpress TdTomato through amplification of GAL4/UAS systemDepositorInsertTdTomato, GAL4
UseXenopus laevis expressionTagsTdTomatoExpressionMammalianMutationPromoteractin-GAL4, UASAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL6
Plasmid#184134PurposeFor labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.DepositorInsertCELE_VW02B12L.1 (vha-6 Nematode)
UseTagsRFPExpressionWormMutationPromoterPvha-6 (894bp)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)
Plasmid#104117PurposesynOptopatch constructDepositorInsertQuasAr3-dark Citrine
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only