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Plasmid#89353PurposeExpresses humanized AsCpf1 variant that recognizes TATV PAMsDepositorInsertCpf1, RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorInsertCas9
UseCRISPR; Xenopus and zebrafish expressionTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-Cetn1/1
Plasmid#50718PurposepX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.DepositorInsertsCetn1 sgRNA1
humanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBh and hU6Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW SpCas9
Plasmid#131506Purposelentiviral expression of SpCas9 under hUBC promotorDepositorInsertSpCas9
UseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT1
Plasmid#210549PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
UseTagsExpressionPlantMutation2bp insertion after AAVS1 target sequence that di…PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9
Plasmid#80425PurposeUsed for in vitro transcription of SpCas9 mRNA.DepositorInsertSpCas9
UseCRISPRTagsnoneExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUbi-MAD7
Plasmid#170133PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-BE4max-BlastR
Plasmid#194061PurposeMammalian expression of evoCjCas9 CBE4maxDepositorInsertevoCjCas9-BE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV1 for Split-PE, SpCas9 nickase (PKC1001)
Plasmid#190111PurposeAAV1 for expression of nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseAAVTagsbpNLSExpressionMutationH840APromoterEF1aAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SaCas9-BlastR
Plasmid#220966PurposeExpresses human codon-optimized SaCas9DepositorInsertSaCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-PBE
Plasmid#98164PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX-evoCas9
Plasmid#107550PurposeExpresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cellsDepositorInsertHumanized S. pyogenes Cas9
UseCRISPRTags3XFLAGExpressionMammalianMutationM495V/Y515N/K526E/R661QPromoterCBhAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDIRECT_23A
Plasmid#91138PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-SaCas9-pU6-sgRNA
Plasmid#159173PurposeVector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neuronsDepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only