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Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-SRF
Plasmid#98618PurposeGateway entry TOPO TA cloning vector for SRFDepositorInsertSRF (Srf Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
Plasmid#136381PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
782 pMEActMEK
Plasmid#31166DepositorInsertMEK (MAP2K1 Human)
UseEntry vectorTagsExpressionMutationSer218Glu Ser222Asp; activated MEKKPromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
L-WARS-26
Plasmid#166528PurposescFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/U6 HDAC4 shRNA
Plasmid#32220DepositorInsertshRNA against murine HDAC4 (Hdac4 Mouse)
UseRNAi; Gateway u6 entry vectorTagsExpressionMutationPromoterhuman U6Available sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR/U6 HDAC5 shRNA
Plasmid#32222DepositorInsertshRNA against mouse HDAC5 (Hdac5 Mouse)
UseRNAi; Gateway u6 entry vectorTagsExpressionMutationPromoterhuman U6Available sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
DT034A
Plasmid#159395PurposeConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
UseSynthetic BiologyTagsFLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…ExpressionBacterialMutationPromoterJ23117 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest IFP1.4
Plasmid#62177PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11
Plasmid#173139PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11 (kcnj11 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only