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Plasmid#121690PurposepMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertcTnT promoter (Cardiomyocyte specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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907L
Plasmid#160237PurposeFlightN-ttgR-VP16-Sv40-attBDepositorInsertsTtgR repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
KG#59
Plasmid#110929PurposeExpresses proteins pan-neuronally in C. elegans (entire nervous system)DepositorInsertsrab-3 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1202
Plasmid#44484DepositorInsertMinimal Mos1, NeoR, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-com-BoxB-vector
Plasmid#132556PurposeExpresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.DepositorInsertsdCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
Lambda N22-KRAB
sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLC-mEos2
Plasmid#38010DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsmEos2ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHD-mW-attPCC-FRT (pTL717)
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP427
Plasmid#87021Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-tdMS2(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLdSaCN
Plasmid#123261PurposeExpresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in LeishmaniaDepositorInsertgRNA and SaCas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy57
Plasmid#122009PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis8 and Multi1: depending on the LP2 primer, eit…ExpressionMammalianMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP mCherry
Plasmid#45233PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD INCENP-Δcen mCherry
UseTagsDNA binding domain of CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPJ173(JPUB_006777)
Plasmid#83834PurposeCarries NEW operon 10 finalDepositorInsertsKuste3336
Kuste3339
Kuste3341
UseTagsExpressionMutationPromoterop10_3339_for, op10_3341_for, and op10_BsaI_3336_…Available sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ174(JPUB_006779)
Plasmid#83827PurposeCarries operon 1 finalDepositorInsertsKuste3603
kuste3605
kuste3606
UsePfab217 (p15a ori, kanr)TagsExpressionMutationPromoterop1_3603_for, op1_3605_for, and op1_3606_forAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS10: pTns(AvCAST)_ΔTnsD
Plasmid#168143PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF257
Plasmid#105497PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiTDepositorInsertSmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-eBFP2
Plasmid#67030PurposePlasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only