Addgene

We narrowed to 17,148 results for: gateway

Showing: 11861 - 11880 of 17148 results

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pAdCMV/V5-DEST-STAT3-3xFlag

Plasmid

#99260

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-hM3Dq

Plasmid

#114283

Purpose

TET-inducible expression of the hM3Dq protein

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

hM3Dq

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-A1Up

Plasmid

#16170

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Ubiquilin 4 (UBQLN4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pLX302 OAS2-V5 puro

Plasmid

#158642

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS2 (OAS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9A(E195Q)-HA

Plasmid

#209244

Purpose

Mammalian expression of ATP9A mutant E195Q

Depositor

Hye-Won Shin

Article

Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

Insert

ATP9A (ATP9A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E195Q

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1

Plasmid

#104980

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 BATF3

Plasmid

#67050

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BATF3 (BATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 S

Plasmid

#158371

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 S (S )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST47-ARL4C-GFP

Plasmid

#67402

Purpose

Mammalian expression of hArl4c with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 NSP5

Plasmid

#158356

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP5 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF1

Plasmid

#167745

Purpose

Expresses ELF1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF1 (ELF1 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Hygro-iNGN3

Plasmid

#75340

Purpose

doxycycline inducible NGN3 gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

NGN3 (NEUROG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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KCNE2_pCSdest

Plasmid

#53848

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

KCNE2 (KCNE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf6

Plasmid

#158374

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf6 (ORF6 )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only