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Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-1st WW mutant
Plasmid#18998DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 199 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ255
Plasmid#124310PurposeGateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editingDepositorInsertrAPOBEC1-pcoCas9D10A-UGI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_Plk5(WT)
Plasmid#136329PurposeHuman Plk5 WT in Gateway pENTRDepositorInsertPLK5 (PLK5 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3x-Flag Gli3 37R3-2
Plasmid#120305PurposeExpresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37CDepositorInsertIntein 37R3-2, Gli3
UseSynthetic BiologyTags3X FlagExpressionMammalianMutationContains mutations as reported in 2011 Chemistry …PromoterCMVAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-2nd WW mutant
Plasmid#18999DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 258 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_EWSR1_His6
Plasmid#26364DepositorInsertEWSR1 (EWSR1 Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 657 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only