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pDONR223-ZNF800

Plasmid

#88573

Purpose

Donor Vector containing ZNF800 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF800 (ZNF800 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Blnk-SH2

Plasmid

#46408

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blnk (BLNK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 333-456

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF219

Plasmid

#88118

Purpose

Donor Vector containing ZNF219 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF219 (ZNF219 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF830

Plasmid

#88342

Purpose

Donor Vector containing ZNF830 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF830 (ZNF830 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ZNF808

Plasmid

#101470

Purpose

Donor Vector containing ZNF808 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF808 (ZNF808 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF331

Plasmid

#88442

Purpose

Donor Vector containing ZNF331 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF331 (ZNF331 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF785

Plasmid

#88459

Purpose

Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF785 (ZNF785 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Promoter

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF232

Plasmid

#88305

Purpose

Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF232 (ZNF232 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Grap2-SH2

Plasmid

#46435

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap2 (GRAP2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 52-159

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

Plasmid

#157693

Purpose

mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGEX Fgr-SH2

Plasmid

#46427

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fgr (FGR Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 137-245

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W

Plasmid

#147902

Purpose

Mammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-H58AF60AA64YV71Y-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W

Plasmid

#147907

Purpose

Mammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR205AH208AR244A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W

Plasmid

#147903

Purpose

Mammalian Expression of HsNot9-R205AH208A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-R205AH208A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W

Plasmid

#147908

Purpose

Mammalian Expression of HsNot9-Y203AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR244A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-pre-Ost1-alphaf(I)-Btl2

Plasmid

#117667

Purpose

Study secretion efficiency of Btl2

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ZNF442

Plasmid

#101455

Purpose

Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF442 (ZNF442 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge