Showing: 11821 - 11840 of 18100 results
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Plasmid#106278PurposeFluorescent fusion protein for FoxO1DepositorInsertsUseTagsClover fluorescent proteinExpressionMammalianMutationDeleted amino acids 401-636, S209A, H212R, S215A,…PromoterEF1a/RPBSAAvailabilityAcademic Institutions and Nonprofits only
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CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE
Plasmid#106303PurposeThe plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imagingDepositorInsertscFv-GCN4-HaloTag-GB1-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
Plasmid#106309PurposeThe plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.DepositorInsert6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
UseTags6xflag-3xHaloTag(MtoL)ExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pBRPB CAG-mCherry-IP
Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCre
Plasmid#106368PurposeN-terminal part of the split-Cre SystemDepositorInsertNCre
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCreERT2
Plasmid#106370PurposeN-terminal part of the split-CreERT2 SystemDepositorInsertNCreERT2
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-ERT2CCre
Plasmid#106371PurposeC-terminal part of the split-CreERT2 SystemDepositorInsertERT2CCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-GFPuv
Plasmid#106382PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-con-GFPuv
Plasmid#106386PurposeConstitutive trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#106396PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγDepositorInsertscas9
Lambda red genes
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and araBADAvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11821 - 11840 of 18100 results