We narrowed to 25,068 results for: crispr
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Plasmid#195299PurposepACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).DepositorInsertTnsABCQ operon of McCAST (Tn7575)
ExpressionBacterialPromoterIPTG inducible lac promoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Sa-gRNA-puro
Plasmid#191652PurposeLentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.DepositorInsertS. aureus gRNAscr
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM159
Plasmid#173215PurposeExpresses dCas9-sfGFP10DepositorInsertdCas9-sfGFP10M2
UseCRISPR and LentiviralExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIBR001
Plasmid#177628PurposeSIBR Intron 1 (Int1) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRExpressionBacterialPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEC626
Plasmid#174367PurposeActivation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.DepositorInsertAlpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
UseSynthetic BiologyExpressionBacterialPromoterJ23106Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC020.306
Plasmid#171148PurposeExpression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133344Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA_cpaA
UseCRISPRPromoterconstitutiveAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVMG0149_Cq-U6-6_2xBbsI-gRNA
Plasmid#169323PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0149_Cq-U6-6_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
Plasmid#169370PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
UseCRISPRExpressionInsectPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VC_Cas1_WT
Plasmid#120364PurposeExpresses wildtype V-C Cas1DepositorInsertVC_Cas1
UseCRISPRExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VD_Cas1_WT
Plasmid#120365PurposeExpresses wildtype V-D Cas1DepositorInsertVD_Cas1
UseCRISPRExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5
Plasmid#117135PurposeExpression vector for Nkx2-5 and gRNADepositorInsertNkx2-5 CDS (Nkx2-5 Mouse)
UseCRISPR and LentiviralMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-Ds
Plasmid#119069PurposeTo clone sgRNA spacer sequence for microinjections in zebrafishDepositorInsertU6a:sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by