We narrowed to 25,046 results for: crispr
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Plasmid#169370PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
UseCRISPRExpressionInsectPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VC_Cas1_WT
Plasmid#120364PurposeExpresses wildtype V-C Cas1DepositorInsertVC_Cas1
UseCRISPRExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VD_Cas1_WT
Plasmid#120365PurposeExpresses wildtype V-D Cas1DepositorInsertVD_Cas1
UseCRISPRExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5
Plasmid#117135PurposeExpression vector for Nkx2-5 and gRNADepositorInsertNkx2-5 CDS (Nkx2-5 Mouse)
UseCRISPR and LentiviralMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-Ds
Plasmid#119069PurposeTo clone sgRNA spacer sequence for microinjections in zebrafishDepositorInsertU6a:sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0039
Plasmid#117606PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)DepositorInsert[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-N
Plasmid#113746PurposeGateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEGFP
UseCRISPR and Gateway CloningTagsmEGFPAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-R4R3-mEGFP-C
Plasmid#113749PurposeGateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR and Gateway CloningTagsmEGFPAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-R4R3-mRuby-C
Plasmid#113755PurposeGateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR and Gateway CloningTagsmRuby2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-R4R3-3xmRuby-C
Plasmid#113757PurposeGateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR and Gateway CloningTags3xmRuby2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106353PurposeCRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locusDepositorInsertD4Z4 gRNA-2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_CELF1
Plasmid#106101PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1DepositorInsertgRNA targeting CELF1 (CELF1 Human)
UseCRISPRAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-tagBFP-KRAB
UseFish expressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9-T2A-mClover3
Plasmid#172293PurposeIn vitro transcription of Cas9-T2A-mClover3 synthetic constructDepositorInsertCas9-T2A-mClover3
UseCRISPRPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAS12069_U6-pegRNA-mCherry
Plasmid#177180PurposepegRNA cloning plasmid with mCherry markerDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTJK644
Plasmid#138012PurposeSIX1 knockoutDepositorAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRha-ABE8e-SpCas9-NG
Plasmid#201190PurposeExpresses the base editor ABE8e-SpCas9-NG in bacterial cellsDepositorInsertABE8e-SpCas9-NG
Tags8xHIS and BP-NLSExpressionBacterialPromoterpRhaBADAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only