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Plasmid#69195Purposecomplementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertKluyveromyces lactis URA3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179012PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10p CAS9 Gal1/10p I-SceI
Plasmid#182519PurposeGalactose-Induced Expression vector for Cas9 and I-SceI.DepositorInsertsI-SceI
Cas9
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastMutationPromoterpTPGI (galactose+aTc inducible)Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mPing
Plasmid#145787PurposemPing Yeast Transposition AssayDepositorInsertmPing
UseTagsExpressionYeastMutationWTPromoterAvailable sinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-tdTomato-hphMX6
Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG1
Plasmid#162602PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG2
Plasmid#162603PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG3
Plasmid#162604PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG4
Plasmid#162605PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS461
Plasmid#89064Purposeleu1 integration plasmid with adh1pr:Z3EVDepositorInsertZ3EV artificial transcription factor
UseLeu1 integrating vectorTagsExpressionMutationPromoteradh1Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAEF5
Plasmid#136305PurposePlasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin FleissDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yAT1.03R122KR126K
Plasmid#132782PurposeNon-binding version of the FRET biosensor for ATP measurements in budding yeastDepositorInsertyAT1.03R122KR126K ymTq2-tdTomato
UseTagsExpressionYeastMutationR122K, R126KPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-TRP1
Plasmid#41599DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags3xHA and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only