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pBLxVi5

Plasmid

#167516

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-Violacein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLuxB

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKCiR5

Plasmid

#149462

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCin

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Expression

Mutation

mutations A36V, R136G

Promoter

LTR

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pBCiG2

Plasmid

#149470

Purpose

Broad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCin

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKNR5

Plasmid

#149466

Purpose

Broad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PSalTTC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF_LuxR

Plasmid

#140039

Purpose

Constutitive expression of LuxR protein.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Insert

pLuxL and LuxR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP704nano

Plasmid

#206190

Purpose

Near-infrared fluorescent protein miRFP704nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTEC18

Plasmid

#30177

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

EBFP2

Expression

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NKX3.1

Plasmid

#42897

Depositor

Edward Gelmann

Article

Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

Insert

NKX3.1 (NKX3-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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RGS-SENP3

Plasmid

#18048

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP3 (SENP3 Human)

Use

Tags

RGS

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Use

Tags

his tag (tev cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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RGS-SENP3m

Plasmid

#18714

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP3m (SENP3 Human)

Use

Tags

RGS

Expression

Mammalian

Mutation

C532A

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
ActTS-GR

Plasmid

#79774

Purpose

FRET-based actinin tension sensors

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

ActTS-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E2

Plasmid

#15788

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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piRFP682-N1

Plasmid

#45459

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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