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PurposeAn empty optimized gRNA expression vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 8122
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenone
- Promoter U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTo construct this vector, pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was modified. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was obtained from Addgene which distributes it on behalf of Wellcome Trust Sanger Institute.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP was a gift from Hiroshi Ochiai (Addgene plasmid # 62348 ; http://n2t.net/addgene:62348 ; RRID:Addgene_62348) -
For your References section:
Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. 10.1093/nar/gkv624 PubMed 26092696