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Addgene

pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
(Plasmid #62348)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62348 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Total vector size (bp) 8122
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    none
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    To construct this vector, pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was modified. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was obtained from Addgene which distributes it on behalf of Wellcome Trust Sanger Institute.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP was a gift from Hiroshi Ochiai (Addgene plasmid # 62348 ; http://n2t.net/addgene:62348 ; RRID:Addgene_62348)
  • For your References section:

    Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. 10.1093/nar/gkv624 PubMed 26092696