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Plasmid#176715PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kluyveromyces lactis (sender part).DepositorArticleInsertmfalpha1Kl
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK005
Plasmid#176709PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter PRM1 of S. cerevisiae (response part)DepositorArticleInsertP-PRM1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK004
Plasmid#176708PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter MSG5 of S. cerevisiae (response part)DepositorArticleInsertP-MSG5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK003
Plasmid#176707PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS3 (response part).DepositorArticleInsertP-FUS3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK002
Plasmid#176706PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS1 of S. cerevisiae (response part)DepositorArticleInsertP-FUS1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Chantico
Plasmid#170659PurposepUC19 vector containing an insertion of the Chantico promoterDepositorInsertChantico
UseCloningTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChantico-Firefly
Plasmid#163901PurposeLuciferase reporter for Chantico lncRNADepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterChanticoAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PKM-Halo
Plasmid#175321PurposeProduction of HaloTag fusion proteinDepositorInsertPKM (PKM Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTN_Halo-STMN1
Plasmid#175299PurposeProduction of HaloTag fusion proteinDepositorInsertSTMN1 (STMN1 Human)
UseCell free expressionTags6xHis-HaloTagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1627
Plasmid#167648PurposeExpress FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C, delta12-16 (TonB box)
UseTagsExpressionBacterialMutationfepA with G54C, delta12-16 (TonB box) mutations (…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1626
Plasmid#167646PurposeExpress FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C, delta12-16 (TonB box)
UseTagsExpressionBacterialMutationfepA with D34C, delta12-16 (TonB box) mutations. …PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235.2
Plasmid#170025PurposeEncodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB238
Plasmid#170033PurposeEncodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB237.2
Plasmid#170032PurposeEncodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW014
Plasmid#131817PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW010
Plasmid#131812PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW008
Plasmid#131809PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW006
Plasmid#131807PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26DepositorArticleInsertMastigocladus laminosus ferredoxin mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG73-β1ARts-sfGFP
Plasmid#102459PurposeExpresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ1AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only