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hum sgk promoter F4R4

Plasmid

#83426

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

Insert

human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

sgk

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter pGL3Basic

Plasmid

#32727

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Tags

Expression

Mutation

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_mito

Plasmid

#113930

Purpose

Plasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

Plasmid

#159583

Purpose

Luciferase reporter for ribosome pausing

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

XBP1u pause site
EIF2S3 5UTR

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2_Renilla_luc-60A

Plasmid

#200397

Purpose

A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Renilla luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_nucl

Plasmid

#113931

Purpose

Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_nucl

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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P427-FBP_13

Plasmid

#139704

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2_Firefly_luc-60A

Plasmid

#200395

Purpose

A plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-OT (wt TCF-4 sites)

Plasmid

#16558

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of wildtype Tcf-4 binding sites

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pGL4.R_TRE_minTK

Plasmid

#211516

Purpose

Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

Depositor

Justin English

Article

Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

SV40 and minTK

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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P305-FBP_4

Plasmid

#139695

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p300 reporter

Plasmid

#11726

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

p300 promoter and 5' UTR (EP300 )

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pGL2-Basic-Myogenin core promoter

Plasmid

#134722

Purpose

Myogenin core promoter in a luciferase reporter plasmid

Depositor

Michael Chin

Article

Sun et al J Biol Chem. 2001 May 25;276(21):18591-6

Insert

Myogenin Promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Myogenin

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Use

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-OF (mutant TCF-4 sites)

Plasmid

#16559

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of mutant Tcf-4 binding sites

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pSPlit-OC-hDib1

Plasmid

#58789

Purpose

Express hDib1-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Use

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pSplit2-NO-PRPF6

Plasmid

#51740

Purpose

Express LucN-PRPF6 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

PRP6 (PRPF6 Human)

Use

Tags

FLAG and Luciferase (1-398aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.27_GRHL-SPE

Plasmid

#177774

Purpose

Luciferase reporter for GRHL

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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