Showing: 1161 - 1180 of 1186 results
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Plasmid#53074Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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pBullet-vac-n
Plasmid#53075Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBullet-vac-c
Plasmid#53076Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
UseTagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS-H2
Plasmid#123358PurposeChimeric ETS domain: H2 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 207 to 215 replaced with corresponding s…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3
Plasmid#124435PurposeChimeric ETS domain: H3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis TagExpressionBacterialMutationResidues 225 to 239 replaced with corresponding s…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-S3
Plasmid#124550PurposeChimeric ETS domain: S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 242 to 246 replaced with corresponding s…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3/S3
Plasmid#124627PurposeChimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 237 to 241 replaced with corresponding s…PromoterAvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCPF101-pfQC-WT
Plasmid#184877Purposeexpresses soluble plasmodium falciparum QC protein with a histagDepositorInsertpfQC-wt
UseTagsHis tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pb-NES-ZapCV5 (cpV143)
Plasmid#112061PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.DepositorInsertNES-ZapCV5
UseTagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCBAAvailabilityAcademic Institutions and Nonprofits only -
TFORF0126
Plasmid#141530PurposeLentiviral vector for overexpressing the CXXC1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCXXC1 (CXXC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only
Showing: 1161 - 1180 of 1186 results