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pSLQ10878
Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10873
Plasmid#183961PurposeU6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPRDepositorInsertsHyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorInsertalpha-catenin FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorInsertFAT FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-ICUE3
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorInsertLyn-ICUE3 (RAPGEF3 Human)
UseTagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS
Plasmid#62861PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertFKBP-ICUE3-NLS
UseTagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-c
Plasmid#53078Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB1 (human) HIS-tag pET
Plasmid#8520DepositorInsertHOXB1 (HOXB1 Human)
UseTagsHis and T7ExpressionBacterialMutationPCR from cDNA clonePromoterAvailable sinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-c
Plasmid#53072Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-c
Plasmid#53074Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
UseTagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cg-c
Plasmid#53070Purposedestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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