Addgene

We narrowed to 3,174 results for: ado

Showing: 2321 - 2360 of 3174 results

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

Plasmid

#118202

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hPGK and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 EGFP-guide3

Plasmid

#118168

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CAMK1D TSS-guide2

Plasmid

#118193

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_VPR:1-96

Plasmid

#211012

Purpose

Mammalian expression of full-length HV1N5 VPR. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

VPR:M1-S96

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBiT3.1-C_TBL1XR1:1-514

Plasmid

#211167

Purpose

Mammalian expression of full-length human TBL1XR1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TBL1XR1:M1-K514 (TBL1XR1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDR45:1-360

Plasmid

#211178

Purpose

Mammalian expression of full-length human WDR45. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR45:M1-F360 (WDR45 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_UNG:1-313

Plasmid

#211093

Purpose

Mammalian expression of full-length human UNG. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

UNG:M1-L313 (UNG Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_STRAP:1-350

Plasmid

#211139

Purpose

Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

STRAP:M1-A350 (STRAP Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_PRPF19:1-504

Plasmid

#211132

Purpose

Mammalian expression of full-length human PRPF19. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PRPF19:M1-L504 (PRPF19 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN-HaloTag_MTA2:1-668

Plasmid

#211016

Purpose

Mammalian expression of full-length human MTA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MTA2:M1-D668 (MTA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBiT3.1-N_RBBP7:1-425

Plasmid

#211113

Purpose

Mammalian expression of full-length human RBBP7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP7:M1-S425 (RBBP7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_NSMAF:1-917

Plasmid

#211292

Purpose

Mammalian expression of full-length human NSMAF. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NSMAF:M1-Y917 (NSMAF Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_TBL1XR1:1-514

Plasmid

#211126

Purpose

Mammalian expression of full-length human TBL1XR1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TBL1XR1:M1-K514 (TBL1XR1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_NUP214:1-2090

Plasmid

#211294

Purpose

Mammalian expression of full-length human NUP214. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NUP214:M1-S2090 (NUP214 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_RFWD3:1-774

Plasmid

#210954

Purpose

Mammalian expression of full-length human RFWD3. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTC-HaloTag_RBBP5:1-538

Plasmid

#211008

Purpose

Mammalian expression of full-length human RBBP5. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP5:M1-L538 (RBBP5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNLF1-C_XPO1:1-1071

Plasmid

#211088

Purpose

Mammalian expression of full-length human XPO1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

XPO1:M1-D1071 (XPO1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_RRP9:1-475

Plasmid

#211133

Purpose

Mammalian expression of full-length human RRP9. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RRP9:M1-S475 (RRP9 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_NUP214:1-2090

Plasmid

#211220

Purpose

Mammalian expression of full-length human NUP214. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

NUP214:M1-S2090 (NUP214 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_DPH7:1-452

Plasmid

#211276

Purpose

Mammalian expression of full-length human DPH7. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DPH7:M1-N452 (WDR85 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR33:1-572

Plasmid

#211253

Purpose

Mammalian expression of human WDR33 fragment M1 to I572. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR33:M1-I572 (WDR33 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_RBBP7:1-425

Plasmid

#211021

Purpose

Mammalian expression of full-length human RBBP7. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP7:M1-S425 (RBBP7 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_RFWD3:1-774

Plasmid

#211103

Purpose

Mammalian expression of full-length human RFWD3. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_RFWD3:1-774

Plasmid

#211108

Purpose

Mammalian expression of full-length human RFWD3. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR82:1-313

Plasmid

#211117

Purpose

Mammalian expression of full-length human WDR82. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR82:M1-D313 (WDR82 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_SMU1:1-513

Plasmid

#211114

Purpose

Mammalian expression of full-length human SMU1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SMU1:M1-P513 (SMU1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_RAE1:1-368

Plasmid

#211227

Purpose

Mammalian expression of full-length human RAE1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RAE1:M1-K368 (RAE1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR48:1-677

Plasmid

#211127

Purpose

Mammalian expression of full-length human WDR48. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR48:M1-T677 (WDR48 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_PALB2:1-1186

Plasmid

#211160

Purpose

Mammalian expression of full-length human PALB2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PALB2:M1-S1186 (PALB2 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTC-HaloTag_WDR91:1-747

Plasmid

#210997

Purpose

Mammalian expression of full-length human WDR91. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR91:M1-A747 (WDR91 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTC-HaloTag_WDR81:1-1941

Plasmid

#210993

Purpose

Mammalian expression of full-length human WDR81. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR81:M1-A1941 (WDR81 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBiT3.1-N_MLST8:1-326

Plasmid

#211216

Purpose

Mammalian expression of full-length human MLST8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MLST8:M1-G326 (MLST8 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_PALB2:1-1186

Plasmid

#211119

Purpose

Mammalian expression of full-length human PALB2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PALB2:M1-S1186 (PALB2 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_TLE1:1-770

Plasmid

#211322

Purpose

Mammalian expression of full-length human TLE1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TLE1:M1-Y770 (TLE1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDHD1:1-1129

Plasmid

#211134

Purpose

Mammalian expression of full-length human WDHD1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDHD1:1-1129

Plasmid

#211175

Purpose

Mammalian expression of full-length human WDHD1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDF3:1-420

Plasmid

#211324

Purpose

Mammalian expression of full-length human WDF3. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDF3:M1-C420 (WDFY3 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDF3:1-420

Plasmid

#211250

Purpose

Mammalian expression of full-length human WDF3. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDF3:M1-C420 (WDFY3 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR77:1-342

Plasmid

#211128

Purpose

Mammalian expression of full-length human WDR77. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR77:M1-E342 (WDR77 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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