Addgene

We narrowed to 11,778 results for: lic

Showing: 1161 - 1180 of 11778 results

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pASTA3

Plasmid

#24657

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Use

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRegev_V2

Plasmid

#204144

Purpose

Single reporter randomly selected from splicing library for biochemical validation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V2 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_V3

Plasmid

#204145

Purpose

Single reporter randomly selected from splicing library for biochemical validation

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

V3 exon in beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PX459-CDKN1A

Plasmid

#217457

Purpose

For CDKN1A (p21) knockout

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for CDKN1A

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Article

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Gm-SceI-pheS

Plasmid

#111609

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Tags

Expression

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_LandingPad

Plasmid

#204186

Purpose

Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Beta globin splicing minigene

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Truncated CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p123shy1-3HA

Plasmid

#67876

Purpose

encodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Use

Expression in ustilago maydis

Tags

HA

Expression

Mutation

Promoter

shy1

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCHERRY8

Plasmid

#24663

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNb-delta S

Plasmid

#107984

Purpose

bacterial expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNb 1038bp-17aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNc-delta S

Plasmid

#107985

Purpose

bacterial expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNc 999bp-16aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Promoter

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVTnT-Flp-3xFLAG

Plasmid

#109167

Purpose

Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

Depositor

Monika Golas

Article

Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

Insert

Flp

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPshRNA2

Plasmid

#126612

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-2

Use

RNAi

Tags

Expression

Mutation

Promoter

Mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-cMYC

Plasmid

#216510

Purpose

Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

cMYC

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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