-
Plasmid#84331PurposeCo-expresses (untagged) Histamine-2 receptor and mCherryDepositorInsertH2R-P2A-mCherry (HRH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mIFP24-C1
Plasmid#54820PurposeLocalization: C1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneUseTagsmIFP24ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pCALNL-Kir2.1
Plasmid#125578PurposeCre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mIFP-Lysosomes-20
Plasmid#56228PurposeLocalization: Lysosome Membrane, Excitation: 683, Emission: 704DepositorInsertLAMP1
UseTagsmIFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pR2Q347y
Plasmid#112680Purposefunctional, fluorescent, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercmVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-EGFP-pHAT
Plasmid#83918Purposehuman Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag IIDepositorInsertDoublecortin isoform b (DCX Human, NM_178152.2)
UseTags6-His and EGFP+Strep-tag IIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3R-P2A-mCherry-N1
Plasmid#84332PurposeCo-expresses (untagged) Histamine-3 receptor and mCherryDepositorInsertH3R-P2A-mCherry (HRH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGExpressionMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinE442Q
Plasmid#134464PurposeM1 SpastinE442Q expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationGlu to Gln at amino acid 442PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase Non-Cleavable
Plasmid#59826PurposeAllows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationNon-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only