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Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorInsertMed25-VBD (MED25 Human)
UseTagsExpressionBacterialMutationinsert is 394-543 onlyPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2 SH2-SH3
Plasmid#46442DepositorInsertGrb2 (GRB2 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 1-217PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF10
Plasmid#88556PurposeDonor Vector containing ZNF10 transcription factor, part of the Human TFome CollectionDepositorInsertZNF10 (ZNF10 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG 17xS/T->A, catalytic active lipin 1
Plasmid#32007DepositorInsertlipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationS106A, S150A, (S281A, T282A), S285A, S287A, S293A…PromoterCMV, SP6Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis
Plasmid#156977PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertSEMA3F (SEMA3F Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SYFP2-CTNNB1
Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #2
Plasmid#174512PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.DepositorInsertplasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
UseTags3xFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(T27N)-HA
Plasmid#79426PurposeExpresses C-terminally HA-tagged Arf6(T27N) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsHAExpressionMammalianMutationchanged Threonine 27 to AsparaginePromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(Q67L)-HA
Plasmid#79425PurposeExpresses C-terminally HA-tagged Arf6(Q67L) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsHAExpressionMammalianMutationchanged Glutamine 67 to LeucinePromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutationPromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP
Plasmid#75118PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRD7PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF768
Plasmid#88524PurposeDonor Vector containing ZNF768 transcription factor, part of the Human TFome CollectionDepositorInsertZNF768 (ZNF768 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-PVR(FL)-FLAG
Plasmid#157622PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertPVR (PVR Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-eGFP-CCR5TC
Plasmid#135528PurposeEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseTagseGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis
Plasmid#156864PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertROBO4 (ROBO4 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-sRGECO
Plasmid#137127PurposeIntersectional viral expression of sRGECO in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX CblB-SH2
Plasmid#46413DepositorInsertCblB (CBLB Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 42-359PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only