-
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeoXTR f0
Plasmid#69157Purposemouse targeting vectorDepositorInsertsDTA
NeoR
GFP
AmpR
UseMouse TargetingTagsExpressionMutationPromoterAmp promoter, PGK, and noneAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-G
Plasmid#164162PurposeExpresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminalDepositorInsertmCherry2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
Plasmid#32479DepositorInsertPSAML141F-GlyR-IRES-GFP
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
cPE
Plasmid#183549PurposeExpresses compact Prime Editor in mammalian cellsDepositorInsertSpCas9 nickase (H840A), Reverse Transcriptase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUF-AsCpf1-pre-gRNA
Plasmid#137849PurposeAsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.DepositorInsertAsCpf1 and pre-gRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionTagsExpressionMutationCodon-optimized for N. crassaPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-SBP-DsRed2-ER-5
Plasmid#170676PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsEPV-SBP-DsRed2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE:shRNA A-pGK:rtTA-Cre
Plasmid#68471PurposeInducible lentivirus expressing an arbitrary shRNA and CreDepositorInsertsGFP-shRNA A-miR30
rtTA-2A-Cre
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterpGK and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Puro-T2A-mCitrine-hSmad2-donor
Plasmid#203663PurposeDonor plasmid for CRISPR knock-inDepositorInsertSmad2
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-nls-NgAgo-GK
Plasmid#86435PurposeExpresses a Flag tag on the N-terminalDepositorInsertNgAgo
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pML3009
Plasmid#193256PurposeGamma-aminobutyric acid sensor expressing eYFP reporterDepositorInsertsgabR
eYFP
UseSynthetic BiologyTagsExpressionMutationPromotersynthetic promoter P2 and synthetic promoter PGab…Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG:: ChR2HA-2a-hM4D
Plasmid#52520PurposeCo-expresses HA tagged channelrhodopsin and hM4DDepositorInsertChR2-2a-hM4D
UseTags2xHAExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1 alpha, and TRE3GAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
UseTagsExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hS + eGFP
Plasmid#74945PurposeDosage control and tracing of reprogramming episomesDepositorInsertSox2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only