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Showing: 11721 - 11740 of 18164 results

  1. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid
    #168038
    Purpose
    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
    Depositor
    Michael Nash
    Article
    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do
    Insert
    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    Use
    Tags
    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168038-plasmid-map-sequence-id-334767

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  3. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  5. pDONR223-ZNF10

    Plasmid
    #88556
    Purpose
    Donor Vector containing ZNF10 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ZNF10 (ZNF10 Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only

  11. BcLOV4 fusion tools screening plasmid #2

    Plasmid
    #174512
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Article
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Insert
    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    Use
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174512-plasmid-map-sequence-id-357202

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  16. INCTbiosyn-p35S-GFP(rc)13

    Plasmid
    #127526
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127526-plasmid-map-sequence-id-250841

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  19. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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Showing: 11721 - 11740 of 18164 results